Abstract
Objective:
To study the predominant calcium-antagonist components of Danshen injection.
Method:
The effects of danshensu, protocatechualdehyde and Danshen injection on calcium concentration in cytoplasm of erythrocytes were examined in vitro by the fluorescent Ca+ -chelator fura-2.
Result:
Either DS182 or PCAD can decrease in dose-dependent cytosolic free calcium concentration in human erythrocytes. They had additive effect when mixed, which was similar to Danshen injection.
Conclusion:
DS182 and PCAD may be predominant calcium-antagonist components of Danshen injection.
MeSH terms
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Adult
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Benzaldehydes / isolation & purification
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Benzaldehydes / pharmacology*
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Calcium / metabolism*
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Catechols / isolation & purification
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Catechols / pharmacology*
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Cytoplasm / metabolism
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Drug Synergism
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Drugs, Chinese Herbal / isolation & purification
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Drugs, Chinese Herbal / pharmacology*
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Erythrocytes / metabolism*
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Female
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Humans
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Injections
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Lactates / isolation & purification
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Lactates / pharmacology*
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Male
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Middle Aged
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Plants, Medicinal / chemistry
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Salvia miltiorrhiza / chemistry
Substances
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Benzaldehydes
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Catechols
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Drugs, Chinese Herbal
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Lactates
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3,4-dihydroxyphenyllactic acid
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protocatechualdehyde
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Calcium