Abstract
A survey of emm gene sequences and an analysis of the pulsed-field electrophoretic profiles of 30 Streptococcus pyogenes isolates with reduced susceptibilities to ciprofloxacin detected the prevalence of isolates with emm type 6 and considerable genetic diversity among isolates. The mechanism of ciprofloxacin resistance in these isolates was based on point mutations in topoisomerase IV subunit C encoded by parC, mainly replacement of serine-79 by alanine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Anti-Infective Agents / pharmacology*
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Antigens, Bacterial / genetics
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Bacterial Outer Membrane Proteins / genetics
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Carrier Proteins / genetics
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Child
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Child, Preschool
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Ciprofloxacin / pharmacology*
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DNA Topoisomerase IV / genetics*
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Drug Resistance, Bacterial / genetics*
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Genetic Variation
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Humans
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Infant
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Microbial Sensitivity Tests
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Pharyngitis / microbiology
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Pharynx / microbiology*
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Point Mutation
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Spain
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Streptococcal Infections / microbiology
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Streptococcus pyogenes / classification
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Streptococcus pyogenes / drug effects*
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Streptococcus pyogenes / genetics
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Streptococcus pyogenes / isolation & purification
Substances
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Anti-Infective Agents
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Carrier Proteins
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streptococcal M protein
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Ciprofloxacin
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DNA Topoisomerase IV