The development and application of automated gridding for efficient screening of yeast and bacterial ordered libraries

Genomics. 1992 Mar;12(3):534-41. doi: 10.1016/0888-7543(92)90445-x.

Abstract

An automated gridding procedure for the inoculation of yeast and bacterial clones in high-density arrays has been developed. A 96-pin inoculating tool compatible with the standard microtiter plate format and an eight-position tablet have been designed to fit the Biomek 1000 programmable robotic workstation (Beckman Instruments). The system is used to inoculate six copies of 80 x 120-mm filters representing a total of approximately 20,000 individual clones in approximately 3 h. High-density arrays of yeast artificial chromosome (YAC) and cosmid clones have been used for rapid large-scale hybridization screens of ordered libraries. In addition, an improved PCR library screening strategy has been developed using strips cut from the high-density arrays to prepare row and column DNA pools for PCR analysis. This strategy eliminates the final hybridization step and allows identification of a single clone by PCR in 2 days. The development of automated gridding technology will have a significant impact on the establishment of fully versatile screening of ordered library resources for genomic studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoanalysis / instrumentation*
  • Autoanalysis / methods
  • Bacteria / genetics*
  • Cloning, Molecular / methods*
  • Cosmids
  • DNA / genetics
  • DNA / isolation & purification
  • DNA Probes
  • Gene Library*
  • Humans
  • Phosphorus Radioisotopes
  • Robotics
  • Saccharomyces cerevisiae / genetics*
  • Sulfur Radioisotopes

Substances

  • DNA Probes
  • Phosphorus Radioisotopes
  • Sulfur Radioisotopes
  • DNA