Phage display vectors for the in vitro generation of human antibody fragments

Michael Hust; Stefan Dübel

doi:10.1385/1-59259-873-0:071

Phage display vectors for the in vitro generation of human antibody fragments

Methods Mol Biol. 2005:295:71-96. doi: 10.1385/1-59259-873-0:071.

Authors

Michael Hust¹, Stefan Dübel

Affiliation

¹ Department of Biotechnology, Institute of Biochemistry and Biotechnology, Carolo-Wilhelmina Technical University, Braunschweig, Germany.

PMID: 15596889
DOI: 10.1385/1-59259-873-0:071

Abstract

A major source of human antibodies are phage display libraries, which are constructed from various genetic sources. Antibodies are expressed as scFV and Fab antibody fragments using various vector systems. This review offers a comprehensive overview of M13 phage display antibody vectors and discusses their applications.

MeSH terms

Antibodies, Monoclonal / genetics*
Bacteriophage M13 / genetics
Cloning, Molecular / methods*
Genetic Vectors / genetics*
Humans
Immunoglobulin Light Chains / genetics*
Immunoglobulin Variable Region / genetics*

Substances

Antibodies, Monoclonal
Immunoglobulin Light Chains
Immunoglobulin Variable Region