[Comparison of five usual techniques for detection of methicillin-resistant Staphylococcus aureus]

Pathol Biol (Paris). 2004 Dec;52(10):617-21. doi: 10.1016/j.patbio.2004.09.005.
[Article in French]

Abstract

The detection of methicillin-resistant Staphylococcus aureus in an usual and daily test in a microbiology laboratory. But its interpretation isn't easy because the resistance expression in vitro is so variable. The authors compare five methods about 100 clinical isolated strains. The results are: disk diffusion method using oxacillin 5 microg, on a NaCl 5% Mueller Hinton agar, 100 ml presentation (biorad): specificity 91%; disk diffusion method using oxacillin 5 microg, on a NaCl 5% Mueller Hinton agar, ready to use plate (AES): specificity 95%; the Oxascreen agar (BD): specificity 96%; the oxacillin cupule of ATB STAPH strip (ATB expression bioMerieux system) NaCl 2% semi solid Mueller Hinton agar medium: specificity 100% and disk diffusion method using cefoxitin 30 microg, on a Mueller Hinton agar plate (bioMerieux): specificity 100%. Therefore the cefoxitin disk diffusion method is very interesting to make better the difference between methicillin-resistant and methicillin-sensible S. aureus, in association with the ATB STAPH strip.

Publication types

  • English Abstract

MeSH terms

  • Cefoxitin / pharmacology
  • Humans
  • Methicillin Resistance / drug effects*
  • Microbial Sensitivity Tests / methods
  • Oxacillin / pharmacology*
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / genetics

Substances

  • Cefoxitin
  • Oxacillin