Expression of epidermal growth factor receptor and c-erbB-2 oncoprotein in trophoblast populations of placenta accreta

Am J Obstet Gynecol. 2004 Dec;191(6):2106-13. doi: 10.1016/j.ajog.2004.04.043.

Abstract

Objective: The purpose of this study was to investigate the expression of epidermal growth factor receptor and c-erbB-2 oncoprotein in trophoblast populations of placenta accreta.

Study design: Paraffin sections of 43 placental specimens with (cases) and 43 without (controls) placenta accreta were studied immunohistochemically for epidermal growth factor receptor and c-erbB-2 expression in the syncytiotrophoblast, villous cytotrophoblast, and extravillous cytotrophoblast. Epidermal growth factor receptor and c-erbB-2 protein levels were also performed by Western blot analysis. Controls were matched for gestational age in this case-control study.

Results: The percentage of strong/intermediate immunoreactivity of epidermal growth factor receptor in the syncytiotrophoblast was significantly higher in cases (98%) than controls (79%; odds ratio,11.1; 95% CI, 1.3-92.0; P = .03), although strong/intermediate immunoreactivity of c-erbB-2 in the syncytiotrophoblast was significantly lower in cases (35%) than controls (81%; odds ratio, 0.1; 95% CI, 0.1-0.3; P < .001). Epidermal growth factor receptor and c-erbB-2 expression in the villous and extravillous cytotrophoblastic cells were not significantly different between controls and cases ( P > .05). Most immunoreactive bands on Western blots were consistent with the trends of immunohistochemical findings.

Conclusion: The development of placenta accreta is related closely to the differential expression of epidermal growth factor receptor and c-erbB-2 in the syncytiotrophoblast.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biomarkers / analysis
  • Biopsy, Needle
  • Blotting, Western
  • Case-Control Studies
  • Confidence Intervals
  • ErbB Receptors / analysis
  • ErbB Receptors / metabolism*
  • Female
  • Gestational Age
  • Humans
  • Immunohistochemistry
  • Odds Ratio
  • Placenta Accreta / pathology*
  • Pregnancy
  • Probability
  • Receptor, ErbB-2 / metabolism*
  • Reference Values
  • Sensitivity and Specificity
  • Tissue Culture Techniques
  • Trophoblasts / pathology*

Substances

  • Biomarkers
  • ErbB Receptors
  • Receptor, ErbB-2