Crystallization of the Lys49 PLA2 homologue, myotoxin II, from the venom of Atropoides nummifer

Leandra Watanabe; Lisandra M Gava; Yamileth Angulo; Bruno Lomonte; Raghuvir K Arni

doi:10.1016/j.bbapap.2004.09.006

Crystallization of the Lys49 PLA2 homologue, myotoxin II, from the venom of Atropoides nummifer

Biochim Biophys Acta. 2004 Dec 1;1703(1):87-9. doi: 10.1016/j.bbapap.2004.09.006.

Authors

Leandra Watanabe¹, Lisandra M Gava, Yamileth Angulo, Bruno Lomonte, Raghuvir K Arni

Affiliation

¹ Department of Physics, IBILCE/UNESP, R. Cristovão Colombo 2265, São José do Rio Preto, SP, CEP 15054-000, Brazil.

PMID: 15588707
DOI: 10.1016/j.bbapap.2004.09.006

Abstract

Myotoxin II, a Lys49 catalytically inactive phospholipase A(2) homologue from Atropoides nummifer venom, was purified, characterized and crystallized. The crystals belongs to the tetragonal system, space group P4(3)2(1)2, with unit cell parameters (a=b=68.66 and c=63.87 angstroms). Diffraction data were collected to a resolution of 2.32 angstroms. The crystal structure is currently being determined using molecular replacement techniques.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bothrops*
Crotalid Venoms / chemistry*
Crystallography, X-Ray
Group II Phospholipases A2
Lysine / chemistry*
Phospholipases A / analysis*
Phospholipases A / chemistry*
Phospholipases A / isolation & purification
Phospholipases A2
Reptilian Proteins
X-Ray Diffraction

Substances

Crotalid Venoms
Reptilian Proteins
myotoxin II, Atropoides nummifer
Phospholipases A
Group II Phospholipases A2
Phospholipases A2
Lysine