Detection of prothrombin and osteopontin in a renal stone found in a hyperuricemic patient using 2D-PAGE and LC-MS analysis

Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(8-9):1127-9. doi: 10.1081/NCN-200027387.

Abstract

The liquid chromatography-mass spectrometry (LC-MS) following on from the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was applied for the analysis of proteins in a renal stone found in a hyperuricemic patient. This technique was sensitive enough to detect small quantities of proteins even in a renal stone.

Publication types

  • Case Reports

MeSH terms

  • Absorption
  • Amino Acid Sequence
  • Chromatography, Liquid / methods
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Hyperuricemia / pathology*
  • Kidney Calculi / metabolism*
  • Male
  • Mass Spectrometry / methods
  • Middle Aged
  • Molecular Sequence Data
  • Osteopontin
  • Prothrombin / analysis
  • Prothrombin / biosynthesis*
  • Sialoglycoproteins / analysis
  • Sialoglycoproteins / biosynthesis*
  • Spectrometry, Mass, Electrospray Ionization

Substances

  • SPP1 protein, human
  • Sialoglycoproteins
  • Osteopontin
  • Prothrombin