Abstract
Aim:
To identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search.
Methods:
The PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA.
Results:
Two unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method.
Conclusion:
PSD technique has greater application prospects in peoteomics.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenocorticotropic Hormone / chemistry*
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Amino Acid Sequence
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Databases, Protein
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Electrophoresis, Gel, Two-Dimensional
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Molecular Sequence Data
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Peptide Fragments / chemistry
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Peptide Mapping
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Ribosomal Proteins / analysis*
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Ribosomal Proteins / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Tissue Plasminogen Activator / chemistry*
Substances
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Peptide Fragments
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Ribosomal Proteins
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ribosomal protein S12
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Adrenocorticotropic Hormone
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Tissue Plasminogen Activator