[Recombinant human bone morphogenetic protein-2 transform NIH3T3 fibroblast to osteo-like cells]

Hua Xi Kou Qiang Yi Xue Za Zhi. 2004 Oct;22(5):420-2.
[Article in Chinese]

Abstract

Objective: To examine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on NIH3T3 cells.

Methods: The NIH3T3 cells were cultured in DMEM with 50 microg/L rhBMP-2. The proliferation ability, alkaline phosphatase (ALP) activity and osteocalcin (OC) quantity of NIH3T3 cells were investigated.

Results: The cells showed decreased proliferation ability but enhanced ALP activity and OC quantity.

Conclusion: 50 microg/L rhBMP-2 can transform NIH3T3 fibroblasts to osteo-like cells.

Publication types

  • English Abstract

MeSH terms

  • Alkaline Phosphatase / drug effects*
  • Alkaline Phosphatase / metabolism
  • Animals
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins / pharmacology*
  • Cell Proliferation / drug effects*
  • DNA / drug effects
  • Fibroblasts / cytology*
  • Mice
  • NIH 3T3 Cells
  • Osteocalcin / drug effects
  • Recombinant Proteins / pharmacology*
  • Transforming Growth Factor beta / pharmacology*

Substances

  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • recombinant human bone morphogenetic protein-2
  • Osteocalcin
  • DNA
  • Alkaline Phosphatase