Dimeric galectin-1 induces IL-10 production in T-lymphocytes: an important tool in the regulation of the immune response

J Pathol. 2004 Dec;204(5):511-8. doi: 10.1002/path.1671.

Abstract

Galectin-1, a beta-galactoside binding protein that can occur as both a monomer and a homodimer, binds to leucocyte membrane antigens such as CD7, CD43, and CD45, and has immune-regulatory functions in several animal models of autoimmune disease. However, its mechanism of action is only partially understood. In this study, a marked increase in IL-10 mRNA and protein levels was demonstrated in non-activated and activated CD4(+) and CD8(+) T-cells, following treatment with a high concentration (dimeric form), but not a low concentration (monomeric form), of recombinant galectin-1 protein. IL-10 is known to suppress TH1 type immune responses and upregulation of IL-10 may thus contribute to the immune-regulatory function of galectin-1. Galectin-1 was strongly expressed on the endothelial cells of human kidney allografts, suggesting a role in the regulation of immune responses in transplantation. Administration of high concentrations of galectin-1 may be a useful tool in the treatment of T-cell-mediated diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / immunology
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Cells, Cultured
  • Endothelial Cells / immunology
  • Galectin 1 / immunology*
  • Graft Rejection / immunology
  • Humans
  • Immunohistochemistry / methods
  • Interferon-gamma / analysis
  • Interferon-gamma / immunology
  • Interleukin-10 / analysis
  • Interleukin-10 / biosynthesis*
  • Kidney / cytology
  • Kidney / immunology
  • Kidney Transplantation / immunology
  • RNA, Messenger / analysis
  • Recombinant Proteins / immunology
  • T-Lymphocytes / immunology*
  • Up-Regulation / immunology

Substances

  • Antigens, CD
  • Galectin 1
  • RNA, Messenger
  • Recombinant Proteins
  • Interleukin-10
  • Interferon-gamma