Analysis of haematopoietic chimaerism by quantitative real-time polymerase chain reaction

Bone Marrow Transplant. 2005 Feb;35(3):283-90. doi: 10.1038/sj.bmt.1704764.

Abstract

Allogeneic bone marrow transplantation (BMT) with marrow ablative conditioning is the treatment of choice for haematopoietic malignancies. The use of nonmyeloablative stem cell transplants has allowed the treatment of patients previously ineligible for BMT because of age or other disease. These reduced conditioning regimes allow the persistence initially of some recipient cells in the blood and bone marrow (haematopoietic chimaerism). Monitoring of the relative proportion of donor and recipient cells is required to assess the success of the procedure, to predict subsequent rejection or impending relapse and to guide the use of donor lymphocyte infusions. We present a quantitative real-time PCR approach for the measurement of haematopoietic chimaerism using the TaqMan. This approach exploits the presence of single-nucleotide polymorphisms (SNPs) to distinguish cells of patient or donor origin. We have designed and validated a panel of seven allele-specific probes to quantify the contribution of patient and donor cells in the haematopoietic population from 12 patient and donor pairs. We have compared the performance of this approach with an existing method and proved it to be superior in both accuracy and sensitivity. The use of more sensitive and accurate techniques permits earlier intervention for improved clinical outcome.

Publication types

  • Case Reports
  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adult
  • Blood Cells
  • Bone Marrow Cells
  • DNA Probes
  • Female
  • Hematopoietic Stem Cell Transplantation
  • Humans
  • Male
  • Microsatellite Repeats
  • Middle Aged
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Single Nucleotide
  • Transplantation Chimera / genetics*
  • Transplantation, Homologous

Substances

  • DNA Probes