Abstract
Odors activate lobster olfactory receptor neurons (ORNs) through phosphoinositide signaling that appears to target a Na(+)-gated nonselective cation channel. The Na(+)-gated channel is a potential member of the growing family of transient receptor potential (TRP) channels. Here, we test the effect of potential antagonists on the channel in cell-free patches from cultured lobster ORNs. We show that the channel is antagonized by H+ and the TRP channel blockers 2-aminoethoxydiphenyl borate, SKF96365, ruthenium red, Al3+, Gd3+, and La3+. We then use this enhanced antagonist profile together with the agonists Na+ and Ca2+ to implicate the channel in signal amplification in the cells.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Calcium / pharmacology
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Calcium Channel Blockers / pharmacology
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Calcium Channels / drug effects
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Calcium Channels / physiology*
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Cations / pharmacology*
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Cells, Cultured
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Dose-Response Relationship, Drug
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Drug Interactions
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Electric Stimulation / methods
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Hydrogen-Ion Concentration
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Imidazoles / pharmacology
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Ion Channel Gating / drug effects
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Ion Channel Gating / physiology*
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Ion Channels / drug effects
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Ion Channels / physiology*
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Membrane Potentials / physiology
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Olfactory Receptor Neurons / drug effects*
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Olfactory Receptor Neurons / physiology*
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Palinuridae / physiology
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Patch-Clamp Techniques / methods
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Probability
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Sodium Chloride / pharmacology
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TRPC Cation Channels
Substances
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Calcium Channel Blockers
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Calcium Channels
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Cations
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Imidazoles
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Ion Channels
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TRPC Cation Channels
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Sodium Chloride
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1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
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Calcium