Alendronate may protect against increased periodontitis-related bone loss in estrogen-deficient rats

J Periodontol. 2004 Sep;75(9):1196-202. doi: 10.1902/jop.2004.75.9.1196.

Abstract

Background: The aim of this study was to evaluate the impact of alendronate (ALD) and estrogen (EST) therapies and their withdrawal on bone loss in experimental periodontitis in ovariectomized rats.

Methods: Eighty-seven Wistar rats were divided into six groups: group 1 (N = 15): sham surgery; group 2 (N = 15): ovariectomy (OVX); group 3 (N = 15): OVX plus alendronate administration for 80 days (AT); group 4 (N = 14): OVX plus alendronate administration for 40 days (AW); group 5 (N = 14): OVX plus 17beta estradiol administration for 80 days (ET); and group 6 (N = 14): OVX plus 17beta estradiol administration for 40 days (EW). Twenty-one days after ovariectomy or sham surgery, one mandibular molar was randomly assigned to receive a ligature, while the contralateral tooth was left unligated. Sixty days later, the animals were sacrificed and the specimens processed.

Results: OVX presented a direct impact on alveolar bone, regardless of plaque accumulation and significantly increased bone loss resulting from periodontitis (P < 0.05). The effect of OVX on unligated sites was significantly reduced by AT, AW, and ET (P < 0.05), but not by EW (P > 0.05). In addition, alendronate administration (AT/AW) significantly reduced the impact of OVX on periodontitis-related bone loss (P < 0.05), while estradiol did not (P > 0.05).

Conclusion: Within the limits of this study, alendronate administration, but not estrogen replacement, may protect against the impact of estrogen deficiency on alveolar bone presenting a significant residual effect after its withdrawal.

MeSH terms

  • Alendronate / therapeutic use*
  • Alkaline Phosphatase / blood
  • Alveolar Bone Loss / prevention & control*
  • Alveolar Process / drug effects
  • Animals
  • Dental Plaque Index
  • Estradiol / therapeutic use
  • Estrogen Replacement Therapy
  • Estrogens / deficiency*
  • Female
  • Furcation Defects / prevention & control
  • Ovariectomy
  • Periodontitis / complications
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Time Factors

Substances

  • Estrogens
  • Estradiol
  • Alkaline Phosphatase
  • Alendronate