Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFNgamma

Vet Immunol Immunopathol. 2004 Dec 8;102(3):291-8. doi: 10.1016/j.vetimm.2004.09.021.

Abstract

In the present study, five eukaryotic double-gene expression plasmids containing porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 and ORF7 genes combined with cDNAs encoding porcine IFNgamma and IL-2 were constructed for evaluation as PRRSV vaccine candidates. After immunization and viral challenge, two of three pigs immunized with pIRESorf5/IFNgamma, one of three pigs immunized with pIRESorf5/IL-2 and one of three pigs immunized with pIRESorf7/IL-2 were protected from lung lesions that were present in other vaccinated and control animals. Virus replication was reduced but not completely prevented in organs of the DNA-vaccinated animals as compared to controls. Therefore, the porcine cytokines IFNgamma and IL-2, delivered in combination with ORF5 or ORF7, may improve the immune efficacy of DNA vaccines against PRRSV.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Antiviral Agents / immunology
  • DNA, Complementary / immunology
  • Interferon-gamma / immunology*
  • Interleukin-2 / immunology*
  • Open Reading Frames / immunology*
  • Plasmids / immunology*
  • Porcine Reproductive and Respiratory Syndrome / immunology
  • Porcine Reproductive and Respiratory Syndrome / prevention & control
  • Porcine respiratory and reproductive syndrome virus / genetics
  • Porcine respiratory and reproductive syndrome virus / immunology*
  • Swine / immunology*
  • Swine / virology*
  • Vaccines, DNA / immunology
  • Viral Vaccines / immunology

Substances

  • Antibodies, Viral
  • Antiviral Agents
  • DNA, Complementary
  • Interleukin-2
  • Vaccines, DNA
  • Viral Vaccines
  • Interferon-gamma