Evidence for a novel splice variant of the alpha subunit of Go in rat male haploid germ cells

Biochem Biophys Res Commun. 1992 Feb 28;183(1):41-7. doi: 10.1016/0006-291x(92)91606-q.

Abstract

Northern analysis shows that Go alpha mRNA is highly expressed in immature germ cells from rat. Whereas alpha o2 mRNA is the major form in pachytene spermatocytes, a message of shorter chain length is present in large amount in haploid germ cells. This mRNA was detected with an oligonucleotide specific for the 3'-coding region of alpha o2, but did not hybridize to oligonucleotides specific for the 5'-untranslated and 5'-coding regions. The results indicate the presence of a novel splice variant of alpha o mRNA, which may code for a Go alpha protein important for germ cell development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • GTP-Binding Proteins / genetics*
  • Genetic Variation
  • Haploidy
  • Male
  • RNA Splicing*
  • RNA, Messenger / genetics*
  • Rats
  • Sertoli Cells / chemistry*
  • Spermatozoa / chemistry*

Substances

  • RNA, Messenger
  • GTP-Binding Proteins
  • GTP-Binding Protein alpha Subunits, Gi-Go