Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Eva Spitalska; Alessandra Torina; Vincenza Cannella; Santo Caracappa; Olivier A E Sparagano

doi:10.1007/s00436-004-1217-2

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Parasitol Res. 2004 Oct;94(4):318-20. doi: 10.1007/s00436-004-1217-2. Epub 2004 Sep 15.

Authors

Eva Spitalska¹, Alessandra Torina, Vincenza Cannella, Santo Caracappa, Olivier A E Sparagano

Affiliation

¹ School of Agriculture, Food and Rural Development, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK.

PMID: 15372232
DOI: 10.1007/s00436-004-1217-2

Abstract

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arachnid Vectors / parasitology*
Cattle
Cattle Diseases / parasitology
DNA, Protozoan / analysis
DNA, Ribosomal / analysis
Deoxyribonuclease HpaII / metabolism
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 18S / genetics
Sheep Diseases / parasitology
Species Specificity
Theileria / classification*
Theileria / genetics
Theileria annulata / classification*
Theileria annulata / genetics
Theileriasis / parasitology*
Ticks / parasitology*

Substances

DNA, Protozoan
DNA, Ribosomal
RNA, Ribosomal, 18S
Deoxyribonuclease HpaII