Comparison of the real-time PCR method and the Gen-Probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens

J Clin Microbiol. 2004 Sep;42(9):4307-9. doi: 10.1128/JCM.42.9.4307-4309.2004.

Abstract

Real-time PCR was compared to Amplified Mycobacterium tuberculosis Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDII were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII.

Publication types

  • Comparative Study

MeSH terms

  • Humans
  • Lung / microbiology*
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification*
  • Organ Specificity
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Tuberculosis / diagnosis*
  • Tuberculosis, Pulmonary / diagnosis*