Abstract
A panel of human monoclonal and recombinant antibody light chains was screened for cleavage of the synthetic peptide corresponding to a neutralizing epitope of hepatitis C virus (residues 192-205 of envelope glycoprotein E1). One of the 39 light chains studied hydrolyzed the Val197-Ser198 bond of the peptide with Km and kcat values of 223 +/- 7 microM and 0.087 +/- 0.001 min(-1).
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal / metabolism
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Catalysis
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Hepacivirus / immunology
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Hepacivirus / metabolism*
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Hepatitis C Antibodies / metabolism*
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Humans
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Hydrolysis
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Immunoglobulin Light Chains / metabolism*
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Peptide Fragments / immunology*
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Peptide Fragments / metabolism*
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Peptide Library
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Recombinant Proteins / metabolism
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Viral Proteins / immunology*
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Viral Proteins / metabolism
Substances
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Antibodies, Monoclonal
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Hepatitis C Antibodies
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Immunoglobulin Light Chains
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Peptide Fragments
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Peptide Library
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Recombinant Proteins
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Viral Proteins