CCR5 targeted SIV vaccination strategy preventing or inhibiting SIV infection

W M J M Bogers; L A Bergmeier; H Oostermeijer; P ten Haaft; Y Wang; C G Kelly; M Singh; J L Heeney; T Lehner

doi:10.1016/j.vaccine.2004.02.050

CCR5 targeted SIV vaccination strategy preventing or inhibiting SIV infection

Vaccine. 2004 Aug 13;22(23-24):2974-84. doi: 10.1016/j.vaccine.2004.02.050.

Authors

W M J M Bogers¹, L A Bergmeier, H Oostermeijer, P ten Haaft, Y Wang, C G Kelly, M Singh, J L Heeney, T Lehner

Affiliation

¹ Department of Virology, Biomedical Research Primate Centre, Rijswik, The Netherlands.

PMID: 15356916
DOI: 10.1016/j.vaccine.2004.02.050

Abstract

Cell-surface CCR5 is a major coreceptor with CD4 glycoprotein, mediating cellular entry of CCR5 strains of HIV-1 or SIV. We targeted the SIV CCR5 coreceptor in a combined CCR5-SIV antigen immunization strategy. Rhesus macaques were immunized i.m. with the 70 kDa heat shock protein (HSP70) covalently linked to the CCR5 peptides, SIV gpl20 and p27. Intravenous challenge with SIV mac 8980 prevented SIV infection or decreased the viral load with the CCR5-SIV combined vaccine. CC chemokines and antibodies which block and downmodulateCCR5 were induced, as well as immune responses to the subunit SIV antigens. This novel vaccination strategy complements cognate immunity to SIV with innate immunity to the CCR5 coreceptor of SIV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Viral / analysis
Antibodies, Viral / biosynthesis
Antigens, Surface / immunology
Antigens, Viral / immunology
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / immunology
Gene Products, rex / genetics
Gene Products, rex / immunology
HSP70 Heat-Shock Proteins / metabolism
Immunization Schedule
Immunoglobulin A / analysis
Immunoglobulin A / biosynthesis
Immunoglobulin G / analysis
Immunoglobulin G / biosynthesis
Kinetics
Macaca mulatta
Membrane Glycoproteins / genetics
Membrane Glycoproteins / immunology
Molecular Sequence Data
Mycobacterium tuberculosis / immunology
Receptors, CCR5 / immunology*
SAIDS Vaccines / therapeutic use*
Simian Acquired Immunodeficiency Syndrome / immunology*
Simian Acquired Immunodeficiency Syndrome / prevention & control*
T-Lymphocytes / immunology
Viral Envelope Proteins / genetics
Viral Envelope Proteins / immunology

Substances

Antibodies, Viral
Antigens, Surface
Antigens, Viral
Gene Products, rex
HSP70 Heat-Shock Proteins
Immunoglobulin A
Immunoglobulin G
Membrane Glycoproteins
Receptors, CCR5
SAIDS Vaccines
Viral Envelope Proteins
gp120 protein, Simian immunodeficiency virus