Amplified fragment length polymorphism (AFLP) was applied to compare 100 isolates of Fusarium poae, Fusarium sporotrichioides, and Fusarium langsethiae. Comparison of fingerprints revealed several markers specific for each group of isolates. Cluster analysis showed a clear separation of F. poae from F. sporotrichioides and F. langsethiae. F. langsethiae isolates formed a rather homogeneous group separated from F. sporotrichioides. The AFLP data obtained support F. langsethiae as a new Fusarium species, for the time being in the section Sporotrichiella.