Abstract
We investigated native structures and mitogenic properties of pokeweed lectin-D isoforms (PL-D1 and -D2) on human peripheral blood lymphocytes along with other isolectins (PL-A to -C). Both native PL-D isoforms appeared to behave as monomers. PL-D2 proliferated the lymphocytes like PL-C, whereas PL-D1 had no mitogenicity. PL-D1 acquired mitogenic activity after trimming of the C-terminal dipeptide.
MeSH terms
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Cell Proliferation
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Dose-Response Relationship, Drug
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Humans
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / drug effects*
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Leukocytes, Mononuclear / immunology
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Lymphocyte Activation / drug effects
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Mitosis / immunology*
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Pokeweed Mitogens / immunology
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Pokeweed Mitogens / pharmacology*
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Protein Isoforms
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Ribosome Inactivating Proteins
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Tetrazolium Salts / pharmacology
Substances
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2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium
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Pokeweed Mitogens
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Protein Isoforms
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Tetrazolium Salts
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pokeweed lectin D
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Ribosome Inactivating Proteins