Surface attachment of nanoparticles using oligonucleotides

Colloids Surf B Biointerfaces. 2004 Apr 1;34(3):165-71. doi: 10.1016/j.colsurfb.2003.12.007.

Abstract

Colloidal polymer particles are widely used in a variety of applications ranging from chromatography to surface modified bioreactors in protein arrays. In the present study, surface attachment of polystyrene particles to a polystyrene substrate has been performed using oligonucleotide hybridization. Thiolated complementary oligomers of cytosine and guanine have been covalently coupled to a pyridyl disulphide (PDS) modified polyethyleneglycol tether, forming part of a triblock copolymer which is adsorbed to the polystyrene surfaces via hydrophobic polypropylene oxide center blocks. The ability to withstand shear forces was studied using a laminar flow cell and the uptake of oligomers on the particles was quantified using two complementary techniques: UV-spectroscopy and sedimentation field flow fractionation. The possibility to tether particles in a flow cell suitable for practical use in e.g. a FIA-system is demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Disulfides / chemistry
  • Microscopy, Electron, Scanning
  • Models, Chemical
  • Nanotechnology / methods*
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemistry*
  • Polyethylene Glycols / chemistry
  • Polymers / chemistry
  • Polystyrenes / chemistry
  • Stress, Mechanical
  • Surface Properties
  • Ultraviolet Rays

Substances

  • Disulfides
  • Oligonucleotides
  • Polymers
  • Polystyrenes
  • Polyethylene Glycols