Human schistosomiasis mansoni: intensity of infection differentially affects the production of interleukin-10, interferon-gamma and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures

Trans R Soc Trop Med Hyg. 2004 Sep;98(9):514-9. doi: 10.1016/j.trstmh.2003.11.009.

Abstract

The effect of the intensity of infection (eggs per gram faeces, epg) on the production of interferon-gamma (INF-gamma), interleukin (IL)-10 and IL-13 by peripheral blood mononuclear cells (PBMCs) from individuals living in a Schistosoma mansoni-endemic area was evaluated. In vitro stimulation of PBMCs with soluble egg antigen (SEA) resulted in significantly higher secretion levels of IFN-gamma in egg-negative individuals compared with those with an intensity of infection of more than 100 epg. In contrast, the egg-positive group produced significantly higher amounts of IL-10. Levels of IL-13 did not differ significantly between egg-positive and egg-negative groups. These findings suggest that IL-10 is an important cytokine in the control of the T helper cell (Th) type 1 responses during human S. mansoni infection, shifting the immune response from Th0 in egg-negative individuals from an endemic area to a Th2 polarization in chronic infected individuals.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Age Factors
  • Animals
  • Antigens, Helminth / immunology*
  • Brazil / epidemiology
  • Cells, Cultured
  • Disease Susceptibility / immunology
  • Endemic Diseases
  • Humans
  • Interferon-gamma / biosynthesis*
  • Interferon-gamma / immunology
  • Interleukin-10 / biosynthesis*
  • Interleukin-10 / immunology
  • Interleukin-13 / biosynthesis*
  • Interleukin-13 / immunology
  • Leukocytes, Mononuclear / immunology
  • Schistosoma mansoni / immunology
  • Schistosomiasis mansoni / epidemiology
  • Schistosomiasis mansoni / immunology*
  • Severity of Illness Index

Substances

  • Antigens, Helminth
  • Interleukin-13
  • Interleukin-10
  • Interferon-gamma