Abstract
By oxidation-reduction assay, the viability of Mycobacterium tuberculosis in sputum specimens was evaluated. The technique is based on the Alamar Blue and Malachite Green dyes, which change their color in response to M. tuberculosis growth. The method is simple, permits visual reading of results, and is applicable for laboratories with limited resources.
MeSH terms
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Color
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Coloring Agents / metabolism
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Culture Media
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Humans
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Mycobacterium tuberculosis / drug effects
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Mycobacterium tuberculosis / growth & development*
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Oxazines*
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Oxidation-Reduction
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Rosaniline Dyes / metabolism
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Sputum / microbiology
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Tuberculosis, Pulmonary / drug therapy*
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Tuberculosis, Pulmonary / microbiology
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Xanthenes*
Substances
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Coloring Agents
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Culture Media
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Oxazines
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Rosaniline Dyes
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Xanthenes
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malachite green
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resazurin