Internal-surface reversed-phase chromatography for plasma metabolite analysis of radiopharmaceuticals

Int J Rad Appl Instrum B. 1992 Aug;19(6):651-7. doi: 10.1016/0883-2897(92)90099-k.

Abstract

The use of internal-surface reversed-phase (ISRP) chromatography of unprocessed plasma samples was investigated as an alternative method of quantitation of the arterial plasma metabolite time course of [18F]N-methylspiperone. The ISRP method was directly compared to standard solid phase extraction/HPLC (SPE/HPLC) methods currently in wide use. Results indicate that: (1) the ISRP method is rapid and minimizes sample preparation; (2) recovery of radioactivity from the ISRP column is greater than 90%; (3) no radioactivity remains associated with chromatographically size excluded proteins and (4) the quantitative results are well correlated with conventional SPE/HPLC methods.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blood Proteins / metabolism
  • Centrifugation / methods
  • Chromatography / methods
  • Chromatography, High Pressure Liquid / methods
  • Evaluation Studies as Topic
  • Fluorine Radioisotopes / blood*
  • Humans
  • Papio
  • Protein Binding
  • Rats
  • Rats, Inbred Strains
  • Spiperone / analogs & derivatives*
  • Spiperone / blood
  • Tomography, Emission-Computed

Substances

  • Blood Proteins
  • Fluorine Radioisotopes
  • Spiperone
  • 3-N-methylspiperone