Objective: To establish and optimize two-dimensional gel electrophoresis technique for proteomics.
Methods: Immobiline pH gradients isoelectric focusing was used as 1D, vertical SDS-PAGE as 2D, some factors such as method and volume of loading sample, choice of gels, concentration of SDS gels, preset of electric program and parameters, protocol for staining the gels were improved.
Results and conclusion: Using the proper method described above, satisfactory 2DE maps of human embryonic lung fibroblast(HLF) and silkworm egg were obtained. 2DE technical platform for proteome research was set up.