[Expression and biological activities of TRAIL fusion protein gene]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003 Nov;19(6):535-7.
[Article in Chinese]

Abstract

Aim: To obtain and express the full-length of TRAIL gene in prokaryocytes.

Methods: The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E.coli.

Results: Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E.coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apoptosis.

Conclusion: The TRAIL-GST with biological activity has been expressed in the prokaryotic system.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Apoptosis Regulatory Proteins
  • Flow Cytometry
  • Glutathione Transferase / genetics
  • HeLa Cells
  • Humans
  • KB Cells
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / physiology
  • Recombinant Fusion Proteins / genetics*
  • TNF-Related Apoptosis-Inducing Ligand
  • Tumor Necrosis Factor-alpha / genetics*
  • Tumor Necrosis Factor-alpha / physiology

Substances

  • Apoptosis Regulatory Proteins
  • Membrane Glycoproteins
  • Recombinant Fusion Proteins
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • Tumor Necrosis Factor-alpha
  • Glutathione Transferase