The efficacy of therapy with albendazole in mice with parasitic meningitis caused by Angiostrongylus cantonensis

Parasitol Res. 2004 Jul;93(4):311-7. doi: 10.1007/s00436-004-1105-9. Epub 2004 Jun 4.

Abstract

Matrix metalloproteinase 9 (MMP-9) is involved in the pathogenesis of parasitic meningitis caused by the nematode Angiostrongylus cantonensis. The present study evaluated the efficacy of albendazole therapy in BALB/c mice infected with the third stage larvae of this nematode. Albendazole showed a pronounced larvicidal activity. Eosinophil numbers significantly increased in infected mice but decreased upon administration of albendazole. Densitometric scanning indicated that albendazole reduced gelatinolytic activity detected by gelatin-substrate zymography. In the cerebrospinal fluid, albendazole reduced the lytic area intensity of the 94 kDa MMP-9 band by 46.5% within 7 days, and by 51.5% by day 14. Examination of brain tissue revealed a similar pattern of decrease (48.6% by day 7, and 53.9% by day 14). Albendazole may thus be an effective compound for the treatment of angiostrongyliasis through its larvicidal activity and facilitation of an improved inflammatory response via the reduction of MMP-9 activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albendazole / therapeutic use*
  • Angiostrongylus cantonensis / drug effects
  • Angiostrongylus cantonensis / enzymology
  • Angiostrongylus cantonensis / physiology*
  • Animals
  • Anthelmintics / therapeutic use*
  • Brain / drug effects
  • Brain / enzymology
  • Brain / parasitology
  • Disease Models, Animal
  • Eosinophils / cytology*
  • Eosinophils / drug effects
  • Immunoenzyme Techniques
  • Larva / drug effects
  • Male
  • Matrix Metalloproteinase 9 / cerebrospinal fluid
  • Matrix Metalloproteinase Inhibitors
  • Meningitis / drug therapy*
  • Meningitis / parasitology
  • Mice
  • Mice, Inbred BALB C
  • Parasitic Diseases, Animal / drug therapy*
  • Parasitic Diseases, Animal / etiology
  • Strongylida Infections / complications
  • Strongylida Infections / drug therapy*

Substances

  • Anthelmintics
  • Matrix Metalloproteinase Inhibitors
  • Matrix Metalloproteinase 9
  • Albendazole