Total oxyradical scavenging capacity of the deep-sea amphipod Eurythenes gryllus

Mar Environ Res. 2004 Aug-Dec;58(2-5):615-8. doi: 10.1016/j.marenvres.2004.03.051.

Abstract

Environmental concern for the deep-sea ecosystem is increasing as contaminants, originating from anthropogenic activities, have been detected in deep-sea biota. However, little is known on the xenobiotics metabolising capability of deep-sea fauna. In this study, the deep-sea amphipod Eurythenes gryllus was selected as sentinel species to measure the total oxyradical scavenging capacity (TOSC). Individuals of E. gryllus were sampled at 2000 m depth in the Arctic Ocean. The TOSC assay was measured on the cytosolic fraction and the soluble fraction (3 kDa) of the digestive gland and on the cell-free haemolymph toward peroxyl, hydroxyl and peroxynitrite radicals according to the method of Winston et al. [Free Radical Biology and Medicine 24 (3) (1998) 480] and Regoli and Winston [Toxicology and Applied Pharmacology 156 (1999) 96]. These results provide the first baseline data set for total antioxidant capacity in a deep-sea amphipod.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amphipoda / metabolism*
  • Animals
  • Arctic Regions
  • Environmental Monitoring*
  • Free Radical Scavengers / metabolism*
  • Hemolymph / metabolism
  • Hydroxyl Radical / metabolism
  • Liver / metabolism
  • Oceans and Seas
  • Peroxides / metabolism
  • Peroxynitrous Acid / metabolism

Substances

  • Free Radical Scavengers
  • Peroxides
  • Peroxynitrous Acid
  • perhydroxyl radical
  • Hydroxyl Radical