Effective method for synthetic peptide immobilization that increases the sensitivity and specificity of ELISA procedures

V S Ivanov; Z K Suvorova; L D Tchikin; A T Kozhich; V T Ivanov

doi:10.1016/0022-1759(92)90326-o

Effective method for synthetic peptide immobilization that increases the sensitivity and specificity of ELISA procedures

J Immunol Methods. 1992 Aug 30;153(1-2):229-33. doi: 10.1016/0022-1759(92)90326-o.

Authors

V S Ivanov¹, Z K Suvorova, L D Tchikin, A T Kozhich, V T Ivanov

Affiliation

¹ Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of Russia, Moscow.

PMID: 1517594
DOI: 10.1016/0022-1759(92)90326-o

Abstract

A procedure is described for the immobilization of synthetic peptide antigens on a plastic solid phase for performing ELISA. The use of a streptavidin-biotinylated peptide system for coating microplates with peptide antigen markedly increased both the sensitivity and the specificity compared to a standard ELISA based on synthetic peptides. The procedure was used for the detection of HIV-1-specific antibodies.

MeSH terms

Amino Acid Sequence
Enzyme-Linked Immunosorbent Assay*
HIV Antibodies / analysis
HIV Seropositivity / immunology
Humans
Molecular Sequence Data

Substances

HIV Antibodies