Viroids: the minimal non-coding RNAs with autonomous replication

FEBS Lett. 2004 Jun 1;567(1):42-8. doi: 10.1016/j.febslet.2004.03.118.

Abstract

Viroids are small (246-401 nucleotides), non-coding, circular RNAs able to replicate autonomously in certain plants. Viroids are classified into the families Pospiviroidae and Avsunviroidae, whose members replicate in the nucleus and chloroplast, respectively. Replication occurs by an RNA-based rolling-circle mechanism in three steps: (1). synthesis of longer-than-unit strands catalyzed by host DNA-dependent RNA polymerases forced to transcribe RNA templates, (2). processing to unit-length, which in family Avsunviroidae is mediated by hammerhead ribozymes, and (3). circularization either through an RNA ligase or autocatalytically. Disease induction might result from the accumulation of viroid-specific small interfering RNAs that, via RNA silencing, could interfere with normal developmental pathways.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Base Sequence
  • DNA / chemistry
  • Gene Silencing
  • Models, Biological
  • Models, Genetic
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Plant Viruses / chemistry*
  • RNA / chemistry*
  • RNA Interference
  • RNA Processing, Post-Transcriptional
  • RNA, Catalytic / chemistry
  • Viroids / chemistry*
  • Viroids / physiology*
  • Virus Replication

Substances

  • RNA, Catalytic
  • hammerhead ribozyme
  • RNA
  • DNA