Abstract
The recently developed whole-column-imaging detection technique for capillary electrophoresis (CE) and capillary isoelectric focusing (CIEF), a commercial whole-column-imaged CIEF instrument and its standard operation protocol are introduced. Furthermore, new developments and applications of whole-column-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.
MeSH terms
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2S Albumins, Plant
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Allergens
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Animals
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Antigens, Plant
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Arachis / chemistry
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Electrophoresis, Capillary / methods
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Glycoproteins / chemistry*
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Green Fluorescent Proteins
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Immunoglobulin G / chemistry*
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Isoelectric Focusing / methods
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Luminescent Proteins / chemistry
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Membrane Proteins
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Phycoerythrin / chemistry*
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Plant Proteins / chemistry*
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Protein Binding / physiology
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Rabbits
Substances
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2S Albumins, Plant
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Allergens
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Antigens, Plant
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Ara h 1 protein, Arachis hypogaea
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Ara h II protein, Arachis hypogaea
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Glycoproteins
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Immunoglobulin G
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Luminescent Proteins
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Membrane Proteins
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Plant Proteins
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Phycoerythrin
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Green Fluorescent Proteins