Aim: To develop a double mAb sandwich ELISA for rapidly and quantitatively detecting galactomannan (GM) antigen of aspergillus fumigatus.
Methods: Four monoclonal antibodies (mAb) against GM of aspergillus fumigatus were used as coating or enzyme conjugated antibodies respectively. Capture and sandwich mAbs were selected by sandwich ELISA paired one by one.
Results: Optimal capture and sandwich mAbs were selected and a highly sensitive double sandwich ELISA established. The sensitivity of detecting GM reached 0.1 microg/L, the detectable range was 0.1-10 microg/L. Coefficient of variation (C.V) obtained from detecting the same sample for 6 times by ELISA was (7.2+/-3.8)%.
Conclusion: A sensitive, repeatable and rapid double mAb sandwich ELISA was established for quantitation of aspergillus fumigatus GM, which might apply to early diagnosis and treatment of patients with aspergillosis.