Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation

FEBS Lett. 2004 May 21;566(1-3):121-5. doi: 10.1016/j.febslet.2004.03.114.

Abstract

While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 degrees C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca(2+) mobilization and mitogen-activated protein kinase-phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • CD3 Complex / chemistry
  • CD3 Complex / metabolism*
  • Calcium / chemistry
  • Calcium / metabolism
  • Cell Membrane / metabolism
  • Cholesterol / deficiency
  • Cholesterol / metabolism
  • Cyclodextrins / pharmacology
  • Cytochalasin D / pharmacology
  • Enzyme Activation
  • Fluorescent Antibody Technique
  • G(M1) Ganglioside / chemistry
  • G(M1) Ganglioside / metabolism
  • Humans
  • Hypothermia, Induced*
  • Jurkat Cells
  • Lymphocyte Activation
  • Mitogen-Activated Protein Kinases / metabolism
  • Prions / metabolism*
  • Protein Structure, Tertiary
  • T-Lymphocytes / metabolism*
  • beta-Cyclodextrins*

Substances

  • Actins
  • CD3 Complex
  • Cyclodextrins
  • Prions
  • beta-Cyclodextrins
  • methyl-beta-cyclodextrin
  • Cytochalasin D
  • G(M1) Ganglioside
  • Cholesterol
  • Mitogen-Activated Protein Kinases
  • Calcium