The effect of phalloidin and jasplakinolide on the flexibility and thermal stability of actin filaments

FEBS Lett. 2004 May 7;565(1-3):163-6. doi: 10.1016/j.febslet.2004.03.096.

Abstract

In this work the effect of phalloidin and jasplakinolide on the dynamic properties and thermal stability of actin filaments was studied. Temperature dependent fluorescence resonance energy transfer measurements showed that filaments of Ca-actin became more rigid in the presence of phalloidin or jasplakinolide. Differential scanning calorimetric data implied that the stiffer filaments also had greater thermal stability in the presence of phalloidin or jasplakinolide. The fluorescence and calorimetric measurements provided evidences that the extent of stabilization by jasplakinolide was greater than that by phalloidin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Actins / chemistry*
  • Animals
  • Antineoplastic Agents / pharmacology
  • Calorimetry
  • Calorimetry, Differential Scanning
  • Depsipeptides*
  • Fluorescence Resonance Energy Transfer
  • Microscopy, Fluorescence
  • Muscle, Skeletal / metabolism
  • Peptides, Cyclic / pharmacology*
  • Phalloidine / pharmacology*
  • Protein Conformation / drug effects
  • Rabbits
  • Temperature
  • Thermodynamics

Substances

  • Actins
  • Antineoplastic Agents
  • Depsipeptides
  • Peptides, Cyclic
  • jasplakinolide
  • Phalloidine