Use of the omp50 gene for identification of Campylobacter species by PCR

J Clin Microbiol. 2004 May;42(5):2301-5. doi: 10.1128/JCM.42.5.2301-2305.2004.

Abstract

We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Base Sequence
  • Campylobacter / classification*
  • Campylobacter / genetics*
  • Campylobacter / isolation & purification
  • Campylobacter coli / classification
  • Campylobacter coli / genetics
  • Campylobacter coli / isolation & purification
  • Campylobacter jejuni / classification
  • Campylobacter jejuni / genetics
  • Campylobacter jejuni / isolation & purification
  • Campylobacter lari / classification
  • Campylobacter lari / genetics
  • Campylobacter lari / isolation & purification
  • DNA, Bacterial / genetics
  • Genes, Bacterial*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Porins / genetics*
  • Sequence Homology, Nucleic Acid
  • Species Specificity

Substances

  • DNA, Bacterial
  • Porins