Detection of reactive oxygen and nitrogen species by EPR spin trapping

Antioxid Redox Signal. 2004 Jun;6(3):619-29. doi: 10.1089/152308604773934387.

Abstract

Electron paramagnetic resonance spin trapping has become an indispensable tool for the specific detection of reactive oxygen free radicals in biological systems. In this review we describe some of the advantages as well as some experimental considerations of this technique and how it can be applied to biological systems to measure oxidative stress.

Publication types

  • Review

MeSH terms

  • Artifacts
  • Electron Spin Resonance Spectroscopy / methods*
  • Free Radicals
  • Kinetics
  • Lipids / chemistry
  • Models, Chemical
  • Oxidative Stress
  • Polymorphism, Genetic
  • Reactive Nitrogen Species / metabolism*
  • Reactive Oxygen Species / metabolism*
  • Spin Labels
  • Spin Trapping / methods*

Substances

  • Free Radicals
  • Lipids
  • Reactive Nitrogen Species
  • Reactive Oxygen Species
  • Spin Labels