Transferrin microheterogeneity in fetal blood

Biol Neonate. 2004;86(2):98-103. doi: 10.1159/000078311. Epub 2004 Apr 30.

Abstract

Objectives: To investigate the distribution of microheterogeneous subfractions of transferrin in fetal blood and the influence of highly sialylated transferrins on fetal growth.

Study method: Serum transferrin concentrations were determined by a standard turbidimetric assay. Microheterogeneous transferrin subfractions were assessed by crossed immunoisoelectric focusing.

Results: In normal term infants, total serum transferrin concentrations and percent distribution of highly sialylated transferrins (> or = 5-sialo-transferrins) were markedly lower; the percent distributions of hyposialylated transferrins (0- and 1-sialo-transferrins) were apparently higher than those in non-pregnant and pregnant women. There was no significant positive correlation between the serum concentrations of total transferrin or highly sialylated transferrins in infants' blood and birth weights (r = 0.187, p = 0.582; r = 0.374, p = 0.257, respectively).

Conclusion: The transferrin microheterogeneity pattern shifted towards reduced glycosylation and sialylation in addition to a decrease in total transferrin concentration in fetal blood compared to that of non-pregnant and pregnant women. The concentrations of serum total transferrin and the highly sialylated transferrins in fetal blood, if higher than a certain level, did not seem to have any influence on normal fetal growth.

MeSH terms

  • Female
  • Fetal Blood / chemistry*
  • Glycosylation
  • Humans
  • Isoelectric Focusing
  • N-Acetylneuraminic Acid / blood
  • Nephelometry and Turbidimetry
  • Pregnancy
  • Transferrin / analysis*
  • Transferrin / chemistry

Substances

  • Transferrin
  • N-Acetylneuraminic Acid