Development of an inducible system to control and easily monitor gene expression in Lactococcus lactis

Plasmid. 2004 May;51(3):256-64. doi: 10.1016/j.plasmid.2004.02.004.

Abstract

This report describes the implementation and use of a maltose-inducible system for regulated gene expression in Lactococcus lactis. The system was established using Green Fluorescent Protein as reporter. The transcription of a gene of interest from the inducible promoter of pLS1RGFP plasmid vector can be easily monitored by fluorescence spectroscopy and microscopy. As an example, the lactococcal ribonuclease III was overproduced in an active form.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • DNA Primers
  • Gene Expression Regulation, Bacterial*
  • Genes, Reporter / genetics
  • Genetic Vectors / genetics*
  • Green Fluorescent Proteins
  • Lactococcus lactis / genetics*
  • Luminescent Proteins
  • Maltose
  • Microscopy, Fluorescence
  • Plasmids / genetics*
  • Ribonuclease III / genetics

Substances

  • DNA Primers
  • Luminescent Proteins
  • Green Fluorescent Proteins
  • Maltose
  • Ribonuclease III