Abstract
While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Birds / virology*
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Cell Line
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Cell Line, Tumor
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Fibroblasts
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Genetic Vectors*
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Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
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Humans
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RNA, Small Interfering / genetics*
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Retroviridae / genetics*
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Retroviridae / physiology
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Virus Replication*
Substances
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RNA, Small Interfering
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Glyceraldehyde-3-Phosphate Dehydrogenases