Abstract
A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.
MeSH terms
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Animals
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Biopsy, Needle / methods
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DNA, Kinetoplast / analysis
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Humans
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Leishmania donovani / genetics
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Leishmania donovani / isolation & purification*
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Leishmaniasis, Cutaneous / parasitology*
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Leishmaniasis, Cutaneous / pathology
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Leishmaniasis, Visceral / complications*
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Leishmaniasis, Visceral / parasitology
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Leishmaniasis, Visceral / pathology
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Polymerase Chain Reaction / methods*
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Skin / parasitology
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Skin / pathology*