Abstract
The prevalence and differentiation of dendritic cells (DC) in lymphoid tissue of simian immunodeficiency virus (SIV)-infected cynomolgus monkeys was studied during disease progression. Lymph node biopsies were consecutively obtained from clinical rapid and slow progressors until the development of disease consistent with simian acquired immunodeficiency syndrome (sAIDS) occurred. Quantitative evaluation of CD1a+ DC and the expression of DC antigens related to maturation (CD83, DC-LAMP and S100b) were performed at the single cell level by in situ image analysis. Despite a persistent prevalence of CD1a+ DC in lymphoid tissue during disease progression, there was a subsequent drop of mature CD83+, DC-LAMP+ and S100b+ DC, correlating with the decline of CD4+ T cells in blood. Thus, disease progression to sAIDS was associated with impaired maturation of DC, and lack of CD83, DC-LAMP and S100b expression.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, CD
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Antigens, CD1 / immunology
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CD83 Antigen
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Cell Adhesion Molecules, Neuronal / immunology
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Dendritic Cells / immunology
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Dendritic Cells / virology*
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Disease Models, Animal*
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GPI-Linked Proteins
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Immunoglobulins / immunology
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Immunohistochemistry
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Lymph Nodes / pathology*
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Lymph Nodes / virology
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Macaca mulatta / immunology*
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Macaca mulatta / virology
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Membrane Glycoproteins / immunology
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Nerve Growth Factors / immunology
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S100 Calcium Binding Protein beta Subunit
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S100 Proteins / immunology
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Simian Acquired Immunodeficiency Syndrome / immunology
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Simian Acquired Immunodeficiency Syndrome / pathology*
Substances
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Antigens, CD
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Antigens, CD1
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CD1a antigen
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Cell Adhesion Molecules, Neuronal
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GPI-Linked Proteins
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Immunoglobulins
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Membrane Glycoproteins
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Nerve Growth Factors
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S100 Calcium Binding Protein beta Subunit
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S100 Proteins
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limbic system-associated membrane protein