PCR amplification of GC-rich templates containing palindromic sequences using initial alkali denaturation

Biotechniques. 1992 Apr;12(4):502-4.
No abstract available

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Cytosine
  • DNA, Viral / analysis
  • Deoxyguanine Nucleotides
  • Edetic Acid
  • Guanine
  • Molecular Sequence Data
  • Nucleic Acid Denaturation*
  • Polymerase Chain Reaction / methods*
  • Rubella virus / genetics
  • Sodium Hydroxide

Substances

  • DNA, Viral
  • Deoxyguanine Nucleotides
  • 2'-deoxy-7-deazaguanosine triphosphate
  • Sodium Hydroxide
  • Guanine
  • Cytosine
  • Edetic Acid