3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis

J Robin Harris; Ulrich Meissner; Wolfgang Gebauer; Jürgen Markl

doi:10.1016/j.micron.2003.10.008

3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis

Micron. 2004;35(1-2):23-6. doi: 10.1016/j.micron.2003.10.008.

Authors

J Robin Harris¹, Ulrich Meissner, Wolfgang Gebauer, Jürgen Markl

Affiliation

¹ Institute of Zoology, Johannes Gutenberg University, D-55099, Mainz, Germany.

PMID: 15036283
DOI: 10.1016/j.micron.2003.10.008

Abstract

Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic "boat shape". A 3D reconstruction from this EM data generated a approximately 3 nm resolution model that correlates well with earlier data of the purported subunit dimer, extracted from the 3D reconstruction of the didecamer of Haliotis tuberculata hemocyanin type 1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dimerization
Hemocyanins / chemistry*
Microscopy, Electron
Mollusca / chemistry*
Protein Subunits

Substances

Protein Subunits
Hemocyanins