Recombinant avian adenovirus CELO expressing the human interleukin-2: characterization in vitro, in ovo and in vivo

L V Cherenova; D Y Logunov; E V Shashkova; M M Shmarov; L V Verkhovskaya; G L Neugodova; D B Kazansky; K K Doronin; B S Naroditsky

doi:10.1016/j.virusres.2003.11.018

Recombinant avian adenovirus CELO expressing the human interleukin-2: characterization in vitro, in ovo and in vivo

Virus Res. 2004 Mar 15;100(2):257-61. doi: 10.1016/j.virusres.2003.11.018.

Authors

L V Cherenova¹, D Y Logunov, E V Shashkova, M M Shmarov, L V Verkhovskaya, G L Neugodova, D B Kazansky, K K Doronin, B S Naroditsky

Affiliation

¹ Gamaleya Research Institute of Epidemiology and Microbiology, Gamaleya Street 18, 123098 Moscow, Russia. cherenova@aport2000.ru

PMID: 15019245
DOI: 10.1016/j.virusres.2003.11.018

Abstract

In our study, a recombinant adenovirus based on the avian adenovirus CELO genome, has been constructed that contains the human interleukin-2 gene. We have shown the production of biologically active recombinant interleukin-2 in vitro (LMH and 293 cells) and in ovo (chicken embryos) infected with recombinant virus CELO-IL2. An increase in the median survival time of C57BL/6 mice carrying B16 melanoma tumors has been demonstrated after multiple intra-tumors injections of the recombinant adenovirus CELO-IL2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chick Embryo / virology
Cloning, Molecular / methods
Female
Fowl adenovirus A / genetics*
Humans
Interleukin-2 / genetics*
Interleukin-2 / therapeutic use
Melanoma, Experimental / drug therapy
Melanoma, Experimental / immunology*
Mice
Mice, Inbred C57BL
Plasmids
Survival Analysis

Substances

Interleukin-2