EI-2346, a novel interleukin-1beta converting enzyme inhibitor produced by Streptomyces sp. E-2346. I. Taxonomy of producing strain, fermentation, isolation, physico-chemical properties, and biological properties

Fumito Koizumi; Atsuhiro Hasegawa; Keiko Ochiai; Katsuhiko Ando; Hidemasa Kondo; Mayumi Yoshida; Yuzuru Matsuda; Satoshi Nakanishi

doi:10.7164/antibiotics.56.985

EI-2346, a novel interleukin-1beta converting enzyme inhibitor produced by Streptomyces sp. E-2346. I. Taxonomy of producing strain, fermentation, isolation, physico-chemical properties, and biological properties

J Antibiot (Tokyo). 2003 Dec;56(12):985-92. doi: 10.7164/antibiotics.56.985.

Authors

Fumito Koizumi¹, Atsuhiro Hasegawa, Keiko Ochiai, Katsuhiko Ando, Hidemasa Kondo, Mayumi Yoshida, Yuzuru Matsuda, Satoshi Nakanishi

Affiliation

¹ Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahimachi, Machida-shi, Tokyo 194-8533, Japan. fumito.koizumi@kyowa.co.jp

PMID: 15015724
DOI: 10.7164/antibiotics.56.985

Abstract

EI-2346, a novel interleukin-1beta converting enzyme (ICE) inhibitor, was isolated from the culture broths of Streptomyces sp. E-2346. EI-2346 selectively inhibited the human recombinant ICE activity with an IC50 value of 3.9 microM, without inhibiting elastase and cathepsin B. EI-2346 also inhibited mature interleukin-1beta secretion from THP-1 cells induced by LPS with an IC50 value of 5.2 microM.

MeSH terms

Caspase Inhibitors*
Cell Line
Culture Media
Enzyme Inhibitors* / chemistry
Enzyme Inhibitors* / metabolism
Enzyme Inhibitors* / pharmacology
Fermentation
Molecular Structure
Naphthoquinones* / chemistry
Naphthoquinones* / pharmacology
Recombinant Proteins / antagonists & inhibitors
Streptomyces / classification*
Streptomyces / metabolism

Substances

Caspase Inhibitors
Culture Media
EI-2346
Enzyme Inhibitors
Naphthoquinones
Recombinant Proteins