Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples

Appl Environ Microbiol. 2004 Mar;70(3):1855-7. doi: 10.1128/AEM.70.3.1855-1857.2004.

Abstract

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(-1) in artificially inoculated bovine feces following enrichment.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Cattle
  • Escherichia coli O157 / genetics*
  • Escherichia coli O157 / isolation & purification*
  • Escherichia coli O157 / pathogenicity
  • Feces / microbiology*
  • Food Microbiology
  • Humans
  • Meat / microbiology
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • Sensitivity and Specificity