PCR for varicella zoster virus genome negative in corneal epithelial cells of patients with Thygeson's superficial punctate keratitis

Eye (Lond). 2004 Mar;18(3):304-5. doi: 10.1038/sj.eye.6700621.

Abstract

Purpose: To find out if varicella zoster virus is the causative agent of Thygeson's superficial punctuate keratitis.

Methods: Epithelial cells were harvested from the punctuate epithelial lesions 9 patients with Thygeson's superficial punctate keratitis. After DNA extraction polymerase chain reaction was carried out with varicella zoster virus primers.

Results: All samples were negative with regard to varicella zoster virus genome.

Conclusions: This result suggests that varicella zoster virus is most probably not the causative agent of Thygeson's superficial punctate keratitis.

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Cornea / virology
  • DNA, Viral / analysis
  • Epithelial Cells / virology
  • Female
  • Herpes Zoster Ophthalmicus / complications*
  • Herpesvirus 3, Human / isolation & purification*
  • Humans
  • Keratitis / virology*
  • Male
  • Middle Aged
  • Polymerase Chain Reaction / methods

Substances

  • DNA, Viral