Nocardia abscessus, a recently established species, was isolated from patients during 2000. In the course of our taxonomic studies on 121 clinical Nocardia isolates in Japan 5 strains isolated from patients plus one strain isolated from soil in Japan, were found to have similar physiological characteristics to those of N. abscessus. Phylogenetic studies using 16S rRNA gene sequence analysis confirmed that these strains belong to N. abscessus. This is the first isolation report of N. abscessus from soil as well as from clinical samples in Japan.